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Understanding Chromatography Resins: A Comprehensive Guide

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Title Tag: Chromatography Resin Guide: Types & Applications Chromatography resins are vital in the world of separation science. They play a key role in purifying biomolecules. These resins are used in various chromatography techniques. Each technique has its unique application and benefits. Resin column chromatography is a popular method. It involves packing resins into a …

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What is Void Volume in Chromatography?

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1. What is Void Volume? The void volume (column void volume or dead volume) is the volume that the mobile phase occupies in a column. For instance, if 80% of the entire column’s capacity is filled up with stationary phase material, the void volume would be 20% of the total column volume. 2. How to Calculate …

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HPLC Column Care and Maintainance

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Ensuring optimum HPLC column performace is important for saving time and cost. The HPLC column peformance and retention characteristics should be monitored at regular intervals using standards. MoniFollowing recommendations are provided for routine HPLC column care and maintainance. Montoring for following signs of potential breakdown in HPLC column performance. A. Signs of a Damaged HPLC …

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