Structured, Mechanistic Diagnostics for HPLC, LC–MS, and Bioanalysis
Modern chromatography problems are rarely simple.
Pressure spikes.
Distorted peaks.
Retention drift.
Unstable LLOQ performance.
Unexpected carryover.
Most troubleshooting relies on memory, scattered notes, or static flowcharts.
The Chromatography Troubleshooting Decision Engine (CTDE) changes that.
What It Is
CTDE is a structured, AI-assisted diagnostic framework built specifically for analytical chemistry.
It converts real-world chromatography expertise into:
- Ranked root-cause analysis
- Mechanistic probability scoring
- Targeted confirmation experiments
- Practical corrective actions
- Preventive SOP guidance
This is not a checklist.
It is a decision engine.
Designed for Real Laboratories
The engine is built for:
- HPLC and UHPLC systems
- LC–MS workflows
- Small molecule bioanalysis
- Method development laboratories
- Regulated environments (GLP / GCP / QC)
Every module is structured around applied laboratory logic — not generic advice.
Available Diagnostic Modules
📈 Peak Tailing Troubleshooter
Separates silanol interaction, column overload, extra-column volume, and injection-solvent mismatch in reversed-phase HPLC — systematically, in nine questions. Use this for method troubleshooting; use the LC–MS PK engine when the assay is bioanalytical.
🔧 High Backpressure Troubleshooter
Diagnoses pressure-related system issues including column blockage, filter restriction, precipitation, and pump-related causes.
🔬 LC–MS PK Peak Integrity Engine
Diagnoses peak tailing, fronting, broadening, and LLOQ distortion in small molecule PK assays.
🧪 AI Method Builder
Draft a starting method from your analyte’s properties — class, polarity, pKa, molecular weight, matrix and detection goal — then refine it. Save methods to your account and export them as PDF. Use this when you are building a method, not troubleshooting one.
How It Works
- You answer a short set of focused questions
- The engine maps responses to diagnostic features
- Hypotheses are weighted and ranked
- You receive structured, actionable guidance
The output includes:
- Mechanistic reasoning
- Confirmation tests
- Corrective actions
- Regulatory impact considerations
Why This Matters
Chromatography troubleshooting is often reactive and inefficient.
CTDE provides:
- Speed
- Structure
- Reproducibility
- Knowledge capture
- Reduced investigation time
It transforms tacit experience into scalable scientific intelligence.