Chromatography troubleshooting is a key part of a chromatographer’s daily work. Here, we present articles and protocols to help a chromatorgher achieve this efficiently.
Chromatography is a powerful tool in chemistry. It helps separate and identify compounds in a mixture. A key concept in chromatography is the RF value. RF stands for Retention Factor. It is a dimensionless number used to describe the movement of a compound on a chromatography medium. Understanding RF values is crucial for interpreting chromatographic …
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Introduction Developing an HPLC method is one of the most fundamental skills in analytical chemistry. Whether working in pharmaceutical analysis, bioanalysis, environmental testing, or QC laboratories, a structured approach to method development saves time, improves reproducibility, and strengthens regulatory defensibility. Yet many scientists still rely on trial-and-error adjustments instead of systematic strategy. This guide outlines …
How to Develop an HPLC Method: A Step-by-Step Practical Guide Read More »
Title Tag: Chromatography Resin Guide: Types & Applications Chromatography resins are vital in the world of separation science. They play a key role in purifying biomolecules. These resins are used in various chromatography techniques. Each technique has its unique application and benefits. Resin column chromatography is a popular method. It involves packing resins into a …
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1. What is Void Volume? The void volume (column void volume or dead volume) is the volume that the mobile phase occupies in a column. For instance, if 80% of the entire column’s capacity is filled up with stationary phase material, the void volume would be 20% of the total column volume. 2. How to Calculate …
Ensuring optimum HPLC column performace is important for saving time and cost. The HPLC column peformance and retention characteristics should be monitored at regular intervals using standards. MoniFollowing recommendations are provided for routine HPLC column care and maintainance. Montoring for following signs of potential breakdown in HPLC column performance. A. Signs of a Damaged HPLC …
A. Introduction Chromatography is an important biophysical technique that enables separation, identification, and purification of components of a mixture for qualitative analysis and quantitative determinations. Three components are central to any chromatography system. Figure 1 demonstrates chromatography principles using a filter paper as a stationary phase for separation of mixture components. This type of chromatography …
A drift in analyte retention time over time is sometimes observed in HPLC chromatograms. This drift takes analytes outside their ‘retention window’ within the data analysis software leading to analyte peaks being missed (Figure 1). Peak drifting is often seen during long analysis sequence, analyzing “dirty” samples, or when installing a new HPLC column. A …
As HPLC chemists we know that columns do have a finite lifetime. You column life time (total number of samples you can run) is dependent on factors such as: the mode of separation the type of packing on the column the type of stationary phase Column care Increase Your Column’s Lifetime While most of these …
Figure 1 shows various peak shape issues and their root causes observed while running an HPLC. These peak shape issues include peak tailing, peak splitting, and peak fronting. In this post, we discuss the root causes for these and their troubleshooting. Poor peak shapes result from factors related to sample, stationary phase, mobile phase, and …
