HPLC LC/MS Troubleshooting

Chromatography troubleshooting is a key part of a chromatographer’s daily work. Here, we present articles and protocols to help a chromatorgher achieve this efficiently.

Understanding Chromatography Resins: A Comprehensive Guide

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Title Tag: Chromatography Resin Guide: Types & Applications Chromatography resins are vital in the world of separation science. They play a key role in purifying biomolecules. These resins are used in various chromatography techniques. Each technique has its unique application and benefits. Resin column chromatography is a popular method. It involves packing resins into a …

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HPLC Column Care and Maintainance

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Ensuring optimum HPLC column performace is important for saving time and cost. The HPLC column peformance and retention characteristics should be monitored at regular intervals using standards. MoniFollowing recommendations are provided for routine HPLC column care and maintainance. Montoring for following signs of potential breakdown in HPLC column performance. A. Signs of a Damaged HPLC …

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What is Chromatography?

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A. Introduction Chromatography is an important biophysical technique that enables separation, identification, and purification of components of a mixture for qualitative analysis and quantitative determinations. Three components are central to any chromatography system. Figure 1 demonstrates chromatography principles using a filter paper as a stationary phase for separation of mixture components. This type of chromatography …

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Drifting HPLC Peak Retention Times

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A drift in analyte retention time over time is sometimes observed in HPLC chromatograms. This drift takes analytes outside their ‘retention window’ within the data analysis software leading to analyte peaks being missed (Figure 1).  Peak drifting is often seen during long analysis sequence, analyzing “dirty” samples, or when installing a new HPLC column. A …

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