Reagent Preparation
Preparation of Ammonium Sulfate Saturated Solution
Introduction Ammonium Sulfate is commonly used for reagent for protein precipitation (ammonium sulfate precipitation) and fractionation as it enhances hydrophobic interactions. Ammonium sulfate is an inorganic sulfate salt obtained by reaction of sulfuric acid with two equivalents of ammonia. A high-melting (decomposes above 280℃) white solid which is very soluble in water (70.6 g/100 g water at 0℃; 103.8 g/100 g water at 100℃). …
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Molarity of 50% (w/w) Sodium Hydroxide (NaOH)
Overview A 50% solution of sodium hydroxide is a clear and colorless liquid. It is available is in premade format from a number of suppliers1-3. It causes severe skin burns and eye damage. The solution may be corrosive to metals. The following are know characteristics of the solution. Density of Sodium Hydroxide = 1.515 g/mlMolecular …
Molarity of 95% (w/w) Sulfuric acid (H2SO4)
Density of H2SO4 = 1.84g/mL Molecular weight of H2SO4 = 98.079 95 ml H2SO4 equals 95 x 1.84 = 174.8 g of H2SO4 Hence, 100 ml 95% H2SO4 volume contains 174.8 g H2SO4. And, 1 liter volume of 95% H2SO4 contains 1748.0 g of H2SO4 So, the molarity of 95% H2SO4 is 1748.0/98.079 = 17.822 …
Molarity of 37% (w/w) Hydrochloric Acid (HCl)
Overview Preparation Step 1: Calculate the volume of 100 grams of Hydrochloric acid. Formula: Density = weight / volume or Volume = weight / density Volume of 100 gram of Hydrochloric acid = 100/1.2 = 83.33 ml Note: 37% (w/w) Hydrochloric acid means that 100 grams of Hydrochloric acid contain 37 …
Molecular and Equivalent Weights of Common Compounds
Chemical Name Molecular Formula Molecular Weight (g/mol) Equivalent Weight (g/equiv) Acetic Acid CH3COOH 60.05 60.05 Ammonium Sulfate (NH4)2SO4 132.14 132.14 Calcium Carbonate CaCO3 100.09 50.05 Citric Acid C6H8O7 192.12 64.04 Citric Acid (monohydrate) C6H8O7. H2O 210.14 70.05 Hydrochloric Acid HCl 36.46 36.46 Lactic Acid C3H6O3 90.08 90.08 Malic Acid C4H6O5 134.09 67.05 Nitric Acid HNO3 …
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Preparation of 0.25 M Sodium Hydroxide (NaOH) Solution
Overview Calculation procedure Parameter Value Required volume 1000 mL Molecular weight of NaOH 39.997 g/mole Concentration of 0.25 M Sodium Hydroxide 0.9999% (% by mass, wt/wt) Preparation Step 1: Find out how many g of Sodium Hydroxide you need. Formula: (0.25 mol/L) * (39.997 g/mol) = 9.9992 g of Sodium Hydroxide in 1 L. So …
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Preparation of 2X YT Media
Overview Reagents Required Equipment Preparation Step 1: To prepare 1000 ml of 2 X YT medium, weigh out 16 g tryptone, 10 g yeast extract, 5 g Sodium chloride (NaCl) in a 2-liter conical flask/beaker. Add 800 ml deionized/Milli-Q water and mix it. The solution will appear translucent-yellowish with undissolved media ingredients. Tip:One can use …