If you’re delving into the world of chromatography, you might have come across a technique called size exclusion chromatography (SEC). This method is essential for separating molecules based on their size, and it plays a crucial role in various scientific fields. In this article, we’ll explore everything you need to know about size exclusion chromatography, …
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Ensuring optimum HPLC column performace is important for saving time and cost. The HPLC column peformance and retention characteristics should be monitored at regular intervals using standards. MoniFollowing recommendations are provided for routine HPLC column care and maintainance. Montoring for following signs of potential breakdown in HPLC column performance. A. Signs of a Damaged HPLC …
As HPLC chemists we know that columns do have a finite lifetime. You column life time (total number of samples you can run) is dependent on factors such as: the mode of separation the type of packing on the column the type of stationary phase Column care Increase Your Column’s Lifetime While most of these …
Figure 1 shows various peak shape issues and their root causes observed while running an HPLC. These peak shape issues include peak tailing, peak splitting, and peak fronting. In this post, we discuss the root causes for these and their troubleshooting. Poor peak shapes result from factors related to sample, stationary phase, mobile phase, and …
