Reagent Preparation
Ethanol
Molecular Formula: C2H6O, C2H5OH CAS: 64-17-5 Synonyms: Ethanol, Alcohol, Ethyl, Grain Alcohol Molecular Weight: 46.07 g/mol Monoisotopic Mass: 46.041864811 Da Exact Mass: 46.041864811 Da Solubility: Greater than or equal to 100 mg/mL at 73 °F Density: 0.79 at 68 °F Viscosity: 1.074 mPa.s at 25 °C References: 1. https://pubchem.ncbi.nlm.nih.gov/compound/Ethanol#section=Ionization-Potential
Preparation of Ammonium Sulfate Saturated Solution
Introduction Ammonium Sulfate is commonly used for reagent for protein precipitation (ammonium sulfate precipitation) and fractionation as it enhances hydrophobic interactions. Ammonium sulfate is an inorganic sulfate salt obtained by reaction of sulfuric acid with two equivalents of ammonia. A high-melting (decomposes above 280℃) white solid which is very soluble in water (70.6 g/100 g water at 0℃; 103.8 g/100 g water at 100℃). …
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Molarity of 50% (w/w) Sodium Hydroxide (NaOH) — Value & Calculator
A 50% (w/w) sodium hydroxide solution is approximately 18.9 M. Using the standard relationship M = (10 × %w/w × density) ÷ molar mass, with density 1.515 g/mL and molar mass 40.00 g/mol: M = (10 × 50 × 1.515) ÷ 40.00 = 18.94 M. Use the calculator below to convert any concentration or plan …
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Molarity of 37% (w/w) Hydrochloric Acid (HCl) — Value & Calculator
Concentrated hydrochloric acid at 37% (w/w) is approximately 12.2 M. Using M = (10 × %w/w × density) ÷ molar mass, with density 1.20 g/mL and molar mass 36.46 g/mol: M = (10 × 37 × 1.20) ÷ 36.46 = 12.18 M. Use the calculator below to convert any concentration or plan a dilution. Molarity …
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Molecular and Equivalent Weights of Common Compounds
Chemical Name Molecular Formula Molecular Weight (g/mol) Equivalent Weight (g/equiv) Acetic Acid CH3COOH 60.05 60.05 Ammonium Sulfate (NH4)2SO4 132.14 132.14 Calcium Carbonate CaCO3 100.09 50.05 Citric Acid C6H8O7 192.12 64.04 Citric Acid (monohydrate) C6H8O7. H2O 210.14 70.05 Hydrochloric Acid HCl 36.46 36.46 Lactic Acid C3H6O3 90.08 90.08 Malic Acid C4H6O5 134.09 67.05 Nitric Acid HNO3 …
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Preparation of 0.25 M Sodium Hydroxide (NaOH) Solution
Overview Calculation procedure Parameter Value Required volume 1000 mL Molecular weight of NaOH 39.997 g/mol Concentration of 0.25 M Sodium Hydroxide 1.00% (w/v) Preparation Step 1: Find out how many g of Sodium Hydroxide you need. Formula: (0.25 mol/L) * (39.997 g/mol) = 9.9992 g of Sodium Hydroxide in 1 L. So to make 1 …
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Preparation of 2X YT Media
Overview Reagents Required Equipment Preparation Step 1: To prepare 1000 ml of 2 X YT medium, weigh out 16 g tryptone, 10 g yeast extract, 5 g Sodium chloride (NaCl) in a 2-liter conical flask/beaker. Add 800 ml deionized/Milli-Q water and mix it. The solution will appear translucent-yellowish with undissolved media ingredients. Tip:One can use …