Agarose: High molecular weight polysaccharide used as a separation medium in biochromatography. It is used in bead form, often in gel-filtration chromatography, with aqueous mobile phases
Absorption: The process of retention in which the solute partitions into a liquid like coating.
Adsorbent: Packing used in adsorption chromatography. Silica gel and alumina are the most frequently used adsorbents in high performance liquid chromatography (HPLC).
Asymmetry: Factor describing the shape of a chromatographic peak. Chromatographic theory assumes a Gaussian shape and that peaks are symmetrical.
Immobilized metal chelate affinity chromatography (IMAC): Proteins and peptides that have an affinity for metal ions can be separated using metal chelate affinity chromatography. The metals are immobilized onto a chromatographic medium by chelation. Certain amino acids, e.g. histidine and cysteine, form complexes with the chelated metals around neutral pH (pH 6–8) and it is primarily the histidine-content of a protein which is responsible for its binding to a chelated metal.
Gas chromatography: Gas chromatography (GC) is a separation technique using gas flow through a glass or metal column that separates compounds based on both volatility and interaction with the liquid stationary phase
Microbore: Refers to the use of smaller than-usual inner diameter columns in HPLC. Columns of 2 mm and less are considered to be microbore sizes. Inner diameters of 0.5 mm and smaller are considered micro-LC columns.
Mobile Phase: A solvent or a gas used to carry analytes across a chromatography column.
Resolution: It denotes the degree of separation between any two peaks. It is defined as the difference between the two retention times divided by their average peak width.
Stationary phase: The immobile phase involved in the chromatographic process where analytes are retained. The stationary phase in LC can be a solid, a bonded, an immobilized or a coated phase on a solid support or a wall-coated phase. The stationary phase often characterizes the LC mode. For example, silica gel is used in adsorption chromatography and octadecylsilane bonded phase is used in reversed phase chromatography.
Thin layer chromatography: Thin layer chromatography (TLC) is an affinity-based method used for separation of compounds. TLC is a very versatile separation method that is widely used for both qualitative as well as quantitative sample analysis. TLC can be used to analyze virtually any substance class. TLC is used by many industries and fields of research, including pharmaceutical production, clinical analysis, industrial chemistry, environmental toxicology, food chemistry, water, inorganic, and pesticide analysis, dye purity, cosmetics, plant materials, and herbal analysis.
UV-Vis Spectroscopy: Ultraviolet-visible (UV-Vis) spectroscopy is a widely used technique for qualitative and quantitative analysis in many areas of science ranging from drug identification, nucleic acid purity checks and quantitation, to quality control in the beverage industry and chemical research.
