Preparation of 2X YT Media
Overview Reagents Required Equipment Preparation Step 1: To prepare 1000 ml of 2 X YT medium, weigh out 16 g tryptone, 10 g yeast extract, 5 g Sodium chloride (NaCl) in a 2-liter conical flask/beaker. Add 800 ml deionized/Milli-Q water and mix it. The solution will appear translucent-yellowish with undissolved media ingredients. Tip:One can use …