The Knowledge Hub
Practical troubleshooting guides for working analytical chemists — HPLC, UHPLC, LC-MS, GC, and reagent prep. Each guide is diagnostic-first and linked to the tool that resolves it.
What is Chromatography?
A. Introduction Chromatography is an important biophysical technique that enables separation, identification, and purification of components of a mixture for qualitative analysis…
Drifting HPLC Peak Retention Times
A drift in analyte retention time over time is sometimes observed in HPLC chromatograms. This drift takes analytes outside their ‘retention window’…
HPLC Column Cleaning
As HPLC chemists we know that columns do have a finite lifetime. You column life time (total number of samples you can…
Poor HPLC Peak Shapes: Troubleshooting
Figure 1 shows various peak shape issues and their root causes observed while running an HPLC. These peak shape issues include peak…