The retardation factor, RF, is the migration coordinate used in planar chromatography. In TLC, HPTLC and paper chromatography it expresses how far the centre of an analyte zone travels relative to the mobile-phase front, under one defined set of conditions. RF is not an intrinsic property of a compound. It is a property of the …
Understanding RF Values in Chromatography Techniques Read More »
A flame ionization detector burns column effluent in a hydrogen–air flame and measures the tiny electrical current produced by the ions that form. The current is proportional to the mass of carbon reaching the flame, which makes the FID the closest thing gas chromatography has to a universal detector for organic compounds. It is also …
GC capillary column care is the control of three failure modes: mechanical damage to the fused-silica tube and its polyimide coating; contamination of the inlet end by nonvolatile sample residue; and chemical degradation of the polysiloxane stationary phase by oxygen and heat. Column life is decided by leak-tight installation, oxygen-free carrier gas, operation within the …
GC Capillary Column Care: Installation, Conditioning, Trimming and Storage Read More »
Gas chromatography troubleshooting is the discipline of working from the symptom pattern of a failing chromatogram, not from a suspected part, to the one subsystem (sample, inlet, pneumatics, column and oven, detector or data system) that explains every symptom with the fewest assumptions, and confirming it with a known standard before anything is replaced. Most …
Gas Chromatography Troubleshooting: Symptoms, Causes and a Diagnostic Path Read More »
