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Flame Ionization Detector

A flame ionization detector burns column effluent in a hydrogen–air flame and measures the tiny electrical current produced by the ions that…

Potassium tert-butoxide

Description: Potassium tert-Butoxide, a tetrameric cubane-like cluster, is used as a non-nucleophilic and strong base in organic chemistry. Details References:

Gas Chromatography (GC) Capillary Columns : Care and Maintenance

The best way to extend the life of a gas chromatography (GC) capillary column is to not foul it in the first…

Preparation of Ammonium Sulfate Saturated Solution

Introduction Ammonium Sulfate is commonly used for reagent for protein precipitation (ammonium sulfate precipitation) and fractionation as it enhances hydrophobic interactions. Ammonium…

What is Chromatography?

A. Introduction Chromatography is an important biophysical technique that enables separation, identification, and purification of components of a mixture for qualitative analysis…

Gas Chromatography Troubleshooting

1. What is gas chromatography Gas chromatography (GC) is a widely used analytical technique for separating, identifying, and quantifying components of complex…

Drifting HPLC Peak Retention Times

A drift in analyte retention time over time is sometimes observed in HPLC chromatograms. This drift takes analytes outside their ‘retention window’…

Molarity of 50% (w/w) Sodium Hydroxide (NaOH)

Overview A 50% solution of sodium hydroxide is a clear and colorless liquid. It is available is in premade format from a…

Chromatography Terms

HPLC Column Cleaning

As HPLC chemists we know that columns do have a finite lifetime. You column life time (total number of samples you can…

Molarity of 37% (w/w) Hydrochloric Acid (HCl)

Overview Preparation Step 1: Calculate the volume of 100 grams of Hydrochloric acid. Formula: Density =  weight / volume or Volume = …